Environmental sustainability has become a global concern as communities continue to experience environmental degradation arising from unsustainable human activities, climate change, poor waste management, deforestation, biodiversity loss, and pollution. While governments and environmental agencies remain the primary actors in environmental governance, Faith-Based Organizations (FBOs) have increasingly emerged as important partners in promoting sustainable environmental practices through advocacy, environmental education, community mobilization, and value-based stewardship. In many African societies, particularly in Nigeria, religious institutions wield considerable influence over individual behaviour and community norms, making them strategic actors in advancing environmental sustainability through eco-spirituality. This study examines the relationship between Faith-Based Organizations, eco-spirituality, and environmental sustainability in Jos Metropolis, Plateau State, Nigeria. Specifically, the study evaluates the extent to which eco-spirituality is integrated into the teachings and activities of FBOs, assesses the impact of FBO-led environmental initiatives on environmental quality and sustainable practices, and determines the relationship between community awareness of environmental sustainability and the contributions of FBOs toward environmental stewardship. The study adopts a mixed-methods research design involving structured questionnaires administered to members of selected Christian and Muslim organizations, complemented by key informant interviews with religious leaders and environmental stakeholders. Quantitative data are analysed using descriptive statistics, Pearson Product Moment Correlation, and multiple regression analysis, while qualitative responses are subjected to thematic analysis. The study is anchored on Stewardship Theory and Ecotheology, which emphasize humanity’s divine responsibility to care for creation. It is anticipated that the findings will demonstrate that eco-spiritual values significantly influence environmental attitudes and behaviours, and that FBOs contribute meaningfully to waste management, tree planting, environmental education, sanitation campaigns, and climate change awareness. The study recommends stronger collaboration between government agencies and FBOs, institutionalization of eco-spiritual education within religious programmes, and greater policy recognition of faith communities as strategic partners in environmental sustainability initiatives.